Protocols
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Making cell culture media/Sterile technique
Making Cell Culture Media/Sterile Technique Protocol
August 2021 - Capers Zimmerman
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Thawing cells
August 2021 - Capers Zimmerman
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Suspension cell culture
Suspension Cell Culture Protocol
August 2021 - Capers Zimmerman
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Adherent cell culture
Adherent Cell Culture Protocol
August 2021 - Capers Zimmerman
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Freezing cells
August 2021 - Capers Zimmerman
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Lentivirus production
Lentivirus Production Protocol
October 2021 - Capers Zimmerman
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Flow cytometry
Flow Cytometry Staining Protocol
May 2023 - Eva Allen
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Retrovirus production and mouse T cell transduction
Retrovirus Production and Mouse T Cell Transduction Protocol
October 2023 - Rob Robino
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Human CAR-redirected T cells and Treg cells
Protocols to generate and test CAR T cells and CAR Tregs in vitro
December 2023 - Capers Zimmerman et al.
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Human CAR T cells in solid tumors
Protocols to test CAR T cells in vivo
December 2023 - Russell Cochrane et al.
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qPCR and human macrophage polarization assay
THP-1 Macrophage Polarization Protocol
December 2024 - Erin O’Connor
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Generation of human CAR Tregs
Video protocol to generate and test CAR Tregs
January 2025 - Russell Cochrane et al.